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To evoke action potentials in LOC efferents, electrical field stimulations (30 V, 5 Hz, 0.5 ms impulse duration, for 3 min) was applied in the 3rd (S 1 ) and 13th (S 2 ) fractions by a Grass S88 stimulator (Grass Instrument Co., Quincy, MA) via platinum electrodes positioned at the upper and lower parts of the tissue chamber