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The protein samples (12 L) and Spectra Multicolor Broad Range Protein Ladder (7.5 L) with 10260 kDa range (Thermo Fisher Scientific) were loaded onto the gel, and electrophoresis was run at 200 V for 22 min in 1 Bolt MES SDS Running Buffer (Invitrogen)
Although healthy females secrete 23 fold greater amounts of GH than males their IGF-1 levels are similar to those of males [36]

& Watts, J

The ideal B12 supplement contains a mix of methylcobalamin, hydroxocobalamin and adenosylcobalamin (11)

In contrast, the Sahara, which is dominated by dust aerosols, exhibits significant differences between the two algorithms
