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Cells 11:127

Folate exists in different forms, including dietary folate (naturally present in foods) and synthetic folic acid found in fortified foods and supplements

The GST construct (Amersham Biosciences) in pGEX-4T-3 vector was purified by manual glutathione-affinity chromatography using Glutathione Sepharose 4 fast flow beads (#17-5132-01, GE Healthcare) and eluted with 10 mM reduced glutathione (#11514, SRL), 150 mM NaCl and 2 mM DTT

To what extent apoptosis in BMECs by MGO can lead to BBB breakdown and BBB leakage in vivo remains, however, unclear

4 J Cell Physiol Mechanical signals and IGF-I gene splicing in vitro in relation to development of skeletal muscle (Cheema et al., 2005)Demonstrates that mechanical strain up-regulates the MGF splice variant in muscle-cell culture, linking loading to the proposed repair signal
