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2.8.2 Clinical studies Increased accumulation of iron in substantia nigra of PD patients was observed with MRI techniques in post mortem studies with significant accumulation in putamen, red nucleus, nucleus caudatus and globus pallidus (Wang et al., 2016) and similar imaging findings on nigrosome imaging were found in cases of PD and parkinsonian syndromes (Li et al., 2022)

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The study protocol was approved by the faculty of medicine of Zagazig University institutional review board (approval number 9350-23-2-2022) before subjects enrollment

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