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Thus, it does that by converting excess fat stores usable energy

doi: 10.3168/jds.2020-19974 2 RadzikowskiDMilczarekAJanochaAOstaszewskaUNiedziaekG

Briefly, worms were collected from NGM plates, washed with 1x phosphate-buffered saline (PBS) buffer (pH 7.4), and re-suspended in 60 l of 1x PBS, 120 l of 2x MRWB buffer (160 mM KCl, 40 mM NaCl, 14 mM Na 2 -EGTA, 1 mM spermidine-HCl, 0.4 mM spermine, 30 mM Na-PIPES [Na-piperazine- N , N -bis (2-ethanesulfonic acid)

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