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This transfer of electrons along the electron transport chain is coupled with the transport of protons across the inner membrane, establishing the electrochemical gradient that generated ATP [42]

Technique B (Angle Method): Insert the needle at a 45-degree angle and inject very slowly (over 20-30 seconds for 2mL), allowing natural pressure equalization to prevent forceful mixing

The rats were given food and water ad libitum and the animal facilities performed routine surveillance for pathogens upon arrival

Mishra and Panda, 2021)

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