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[DOI] [PMC free article] [PubMed] [Google Scholar] 15.Tsugane S

Safety / Best Practices Be careful when handling needles and reconstituting vials, as improper sterile technique can lead to dangerous infections

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14 PCR amplification was performed in a final volume of 25 l of the reaction mixture containing 1.5 U of Taq DNA polymerase (Sibenzyme), about 1 g DNA of each patient's DNA, 0.25 M each primer, 250 M of dNTPs, 2 mM of MgCl 2 , 1 PCR buffer of the following composition: 67 mM Tris-HCl pH 8.8, 16.6 mM (NH 4 ) 2 SO 4 and 0.01% Tween-20

doi: 10.1089/gtmb.2017.0010 113