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doi: 10.1016/j.addr.2021.113864 180 YangL.LiuY.WuH.SongZ.HibyN.MolinS.et al

For the assessment of kidney injury severity (severity score), HE stained section images at 10 and 20 (2 at each magnification, for a total of 4 images per mouse) were graded from 0 (no damage) to 5 (most severe damage observed across the samples analyzed), based on the following parameters: (i) protein cast formation, (ii) visible tubular dilation, (iii) loss of proximal tubular cell polarization (no visible brush-border membranes) (iv) area affected (with single nephron damage to majority of the cortex with visual signs of injury)