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Description
Plant Cell Tissue Org Cult
At physiological pH, the thiol residue of GSH is scarcely deprotonated (pK a =9.2)
Non-consideration of these long-known and well-recognized pitfalls will inevitably lead to artificial oxidation of GSH to GSSG thus generating invaluable biased GSH/GSSG ratios, falsely cheating altered oxidative stress
The latter process immobilizes into ATP the chemical free energy generated by the successive steps of the mineralization of the carbon substrates of glycolysis and of the Krebs cycle occurring in the mitochondria
[DOI] [Google Scholar] Nani A
