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Description
This solution contained (in mM): 120 CsF, 10 CsCl, 10 EGTA, 10 HEPES, 0.1 CaCl 2 , 1 BAPTA to achieve calculated free [Ca 2+ ] of 13 nM
Covers supplies, concentration math, step-by-step mixing, storage, and common mistakes
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At this rate, it is expected that there will be 1 billion people worldwide with obesity by 2025