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Signs It Should Be Discarded Cloudiness or floating particles Discoloration Expired beyond 28 days post-opening Broken seal or cracked vial Storage Best Practices To ensure bacteriostatic water remains sterile and safe for injection: Store between 20C to 25C (68F to 77F) Avoid direct sunlight or extreme heat Keep the vial sealed when not in use Always use a clean syringe for each draw Bacteriostatic water is effective for up to 28 days after opening, assuming it's stored and handled properly

The IC 50 was calculated in GraphPad Prism

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The melanocortin action is biased toward protection from weight loss in mice