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After thoroughly mixing the extract by vortexing, the precipitated protein was removed by centrifugation (16,000 g , 5 min, 4 C) before the supernatants were analysed by HPLCMS

39,40 In addition to delivering Cu to the secretory compartment and cytosolic proteins, Cu is targeted to the mitochondria, where cytochrome oxidase (COX) uses Cu for oxidative phosphorylation and mitochondrial function

Tian and his team (109) enhanced the intrinsic and broad-spectrum catalytic reactive oxygen scavenging activity of Co 3 O 4 nanocrystals, named Mn-Co 3 O 4 , by modulating its electronic structure

However, translational research involving humans is scarce

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