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doi: 10.3389/fimmu.2022.812774, PMID: [DOI] [PMC free article] [PubMed] [Google Scholar] Franco A

The percentage of disulfiram-treated patients who completed these trials, ranging from eighteen to eighty-five percent, was higher for those populations in which drug administration was supervised by clinic staff or a family member

Banks and Kastin reported that radiolabeled DSIP administered peripherally produced detectable DSIP-like immunoreactivity in brain tissue at levels above what non-specific leakage would predict, and that the modified analogue [D-Ala3]-DSIP entered brain at substantially higher levels than the parent compound after intracarotid administration (Banks & Kastin, 1981)

Following washing in tris-buffered saline (TBS) containing 0.1% tween 20, the membrane was incubated with a primary antibody against His-Tag (1 g/mL, Novagen) overnight at 4 C