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The behavioral withdrawal signs indicative of the rats having become physically dependent on morphine, such as jumping, wet-dog shakes, rearing, fore-paw licking, circling, writhing, and sneezing (rapid expulsions of air), as well as decreases in body weight and body temperature, were consistent with previous reports published using this same slow-release morphine model (Lee and Fennessy, 1970

Furthermore, whilst loss of C9orf72 in mice models disturbs microglial function, resulting in age-related neuroinflammation, this was not sufficient to cause neurodegeneration in C9orf72 knockout mice (Lall and Baloh, 2017)

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