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[8] Substrates [edit] While CROT's canonical substrate is octanoyl-CoA, CROT is also known to be able to catalyze the deacylation of numerous acyl-CoAs, such as acetyl-CoA, propionyl-CoA, butyryl-CoA, and hexanoyl-CoA

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A medically supervised tapering process is recommended to gradually reduce hormone intake over a few weeks, helping to manage withdrawal symptoms and stabilize hormone levels

doi: 10.1007/s12017-007-8005-y

These include biophysical assays such as surface plasmon resonance and isothermal titration calorimetry to differentiate specific binding from nonspecific aggregation (Kenakin, 2019)