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Blood samples were obtained from 116 children hospitalised at the Department of Paediatric Haematology and Oncology (Faculty Hospital Motol, Prague, Czech Republic) with newly diagnosed solid tumours of neuroblastoma (n=27), nephroblastoma (n=8), anaplastic ependymoma (n=4), Ewings sarcoma (n=9), germ cell tumour (n=4), osteosarcoma (n=16), tumour of the genital tract (n=6), Hodgkins lymphoma (n=16), lymphadenopathy (n=3), medulloblastoma (n=15), rhabdomyosarcoma (n=4) and retinoblastoma (n=4)

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Additionally, AOx enzymes namely, SOD, CAT and GPX were significantly elevated, collectively enhancing free radical scavenging and reducing oxidative damage [75]

Although the silane groups of the C18 column are also fully protonated the acidic environment provides sufficient protons (H + ) in the solution that the acidic NAC will retain its protons, improving retention on RP-HPLC

The cellular metabolic mechanisms of ferroptosis are illustrated in Figure 1