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(2018) 13:e0205708

Membranes were incubated with primary antibodies diluted in 5% milk 1X TBST overnight at 4C: anti-Actin (1:3000) (Sigma, A2066), anti-NRF2 (1:1000) (Cell Signaling Technology #12721) and anti-GSR (1:1000) (ThermoFisher, PA5-29945), followed by washing and incubation with HRP-conjugated anti-rabbit IgG (1:2000) (CST#7074) in 5% milk 1X TBST (22C, 1 h)

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We have demonstrated that EGCG at subinhibitory concentrations (1550 g/mL) reverses tetracycline resistance in staphylococci by inhibiting the Tet(K) efflux pump, leading to increased intracellular tetracycline accumulation [91]