gst-glutathione affinity purification Transglutaminase 2 crosslinks the glutathione S-transferase tag, impeding protein–protein interactions of the fused protein Glutathione-S-Transferase (GST)-Fusion Based Assays for

Hu C, Jiang L, Geng C, et al

Read, G

The staining solution, containing 5 ml DMEM, 8 l FDA (5 mg/ml) and 50 l PI (2 mg/ml) was added to the wells, cells were incubated at room temperature for 4-5 minutes in the dark, washed with PBS and examined on Eclipse Ti-E confocal laser scanning microscope (CLSM) with A1 confocal module (Nikon Corporation, Japan) and CFI Plan Apo VC 20/0.75 objective lens with a life support system, maintaining normal culturing conditions (37C, 5% CO 2 )

DAG helps activate protein kinase C (PKC), which phosphorylates many other proteins, changing their catalytic activities, leading to cellular responses

Collagen synthesis pathways demonstrate coordinated regulation under dual compound treatment