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Many studies have also shown that Zn deficiency decreases the activity of bone-related enzymes and minerals such as ALP, Ca, P and Mg
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Since no commercial cystine-free IMDM is available, we customized IMDM based on a DMEM medium (#D9800-28, USBiological, Salem, MA) without amino acids, glucose, sodium bicarbonate, and sodium pyruvate
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