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The cleared lysate was then mixed with nickel-nitrilotriacetic acid (Ni-NTA)-agarose beads (QIAGEN, Hilden, Germany) or glutathione (GSH) Sepharose 4B beads (Cytiva, Marlborough, MA, USA), and incubated at 4 C for 1 h with rotation to collect tagged proteins

Biomolecules 13 , (2023)

Interleukin-6 as an early chronic inflammatory marker in polycystic ovary syndrome with insulin receptor substrate-2 polymorphism

Indeed, the most recognized effect of NO on skeletal muscle metabolism is its capacity to directly inhibit mitochondrial electron transport chain complexes: NO inhibits electron transfer and NADH-dehydrogenase function at the level of Complex I through the intra-mitochondrial production of ONOO [67]

Endogenously, succinylated KEAP1 dissociates from the NRF2 protein to help cancer cells survive stress