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Immunofluorescence staining For most of the immunostaining experiments, cells were fixed with ice-cold methanol for 5 min at 20, washed three times with PBST (0.1% TritonX-100 in PBS) for 5 min and incubated for 30 min with 1% BSA at room temperature

Multiple facets of intestinal permeability and epithelial handling of dietary antigens

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The CBLIF gene is located on chromosome 11q12.1 and is composed of 9 exons that encode a 417 amino acid precursor protein
