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The solation of alginate cores was verified by labelling the alginate cores with rhodamine 123 and observing the diffusion of the dye into buffer when melting the agarose gel shells under the presence of EDTA whereas the alginate cores remained in a gel form with 50 mM CaCl 2 (Supplementary Fig

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In COPD, this balance is disruptedprimarily manifested as enhanced protease activity and diminished antiprotease activity ( 1-antitrypsin (AAT) represents the most active antiprotease in the lungs, capable of binding to proteases such as neutrophil elastase (NE) at a 1:1 molar ratio to mitigate tissue damage

After centrifugated and washed with PBS, the cells were transferred into RPMI-1640 liquid medium (the treated group contained 5 mmol/L H 2 O 2 ), and cultured for 4 h at 37 C to induce hyphal growth