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The healing process occurs immediately after detachment from both sides: the muscle and the bone
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The entire pool of ~340,000 unique mutants was inoculated in LB medium at OD 600 0.025 (~75 CFU per mutant) and allowed to reach early exponential phase (OD 600 0.15)

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On a U-100 insulin syringe (the standard 1 mL syringe with 100 unit gradations), 10 units delivers 1 mg, and 20 units delivers 2 mg