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For example, the concentration of methyltetrahydrofolate (the principal circulating form of folate) in the brain is four times that seen in plasma [39], whereas biotin and pantothenic acid exist in the brain at concentrations of up to 50 times that seen in plasma [41]

Exogenous dosing amplifies that signal

Gustavsson EK, Zhang D, Reynolds RH, Garcia-Ruiz S, Ryten M

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Some physicians limit B12 shots only to those with documented deficiencies or absorption issues to prevent unnecessary treatment where dietary B12 is sufficient