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In a second experiment, we evaluated two non-teratogens in combination with the maximum tolerated DMSO concentration of the first experiment, as very recently combined toxic effects of DMSO with chemicals that are non-toxic by themselves have been reported ( 2 Materials and Methods 2.1 Chemicals and Solutions Embryo medium was made by dissolving 0.60 g of Instant Ocean Sea Salt (Blacksburg, VA, United States) and 0.038 g of sodium bicarbonate (Sigma, Diegem, Belgium) in 2 L reverse osmosis (RO) water (pH 7.4 0.3) (Barnstead Pacific RO Water Purification System, Thermo Scientific, Waltham, MA, United States )