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One way of assessing drug synergy consists of combining drugs at half of their IC50 concentration for a two-drug combination, and one-third of their IC50 concentration for a three-drug combination (70)

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Mice in the vehicle cohort received three subcutaneous (SC) saline (1 ml/kg) injections/day (~0930, 1330, 1730 h) and mice in the treatment cohort received three SC injections of the NNMT inhibitor 5-amino-1MQ at a dose of 20 mg/kg/injection for a total dose of ~34 mg/kg/day of the parent compound (calculated according to free weight) for 11 days