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Western blot results showed that high glucose reduced the expression of Sirt3, FoxO3a, nuclear factor erythroid 2-related factor 2 (Nrf2), NADPH oxidase 2 (NOX2), and other related proteins, while curcumin significantly increased their expression compared to high-glucose

Although composite models better recapitulate disease progression, their complexity, long duration, and high cost severely restrict their use as mainstream platforms for high-throughput screening and mechanistic studies

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