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Elevated pigment epithelium-derived factor induces diabetic erectile dysfunction via interruption of the Akt/Hsp90/eNOS complex
[DOI] [PubMed] [Google Scholar] 6.Hu L., Zhao Y., Xu H

The next day the membrane was washed three times 10 min with tTBS then incubated 1 h at RT with the secondary antibody (NA934, GE Life Science, USA) diluted 1:5000
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This results in the involvement of all of the polar groups of the molecule in the hydrogen bonding, so that none are available for hydration