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Each sample was spread on SCHisLeuUra+Ade agar plates, scraped, inoculated into a 1.5 ml tube containing 500 l of SCHisLeuUra+Ade medium and cultured for 24 days at 30 C

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( Suppliers of DMEPOS meeting the requirements of sections 1834(a)(20) and 1834(j) of the Act, and related implementing regulations at 42 CFR 424.57 must enroll in Medicare or change their enrollment using the paper application Medicare Enrollment Application for DMEPOS Suppliers (CMS-855S) or through the Medicare Provider Enrollment, Chain, and Ownership System (PECOS)