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The plate was then incubated for 5 min at room temperature before measuring the absorption at 590 nm using the Fluostar Optima (BMG LabTech, Ortenberg, Germany)

Simultaneously, astrocytes respond to chronic hypoxic stress by undergoing reactive astrogliosis, characterized by elevated HIF-1 and HIF-2 signalling and secretion of VEGF-A, which disrupts tight-junction proteins such as occludin and claudin-5

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Its produced primarily in the liver forming the enzyme glutathione peroxidise and is important for many aspects of cellular function

Thus, research is focused on the development of novel treatment approaches