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Also, all other mutations found at this position in high replicator ReEDs presented a hydrophobic amino acid (P2209A/V/I) and enhanced RF to a various extent, but less efficiently than P2209L (Fig

58,60,61 RNA isolation and quantitative RTPCR analysis Total RNA was extracted via TRIzol (Invitrogen), reverse-transcribed into cDNA via a Monad RT Kit, and amplified via SYBR Green Master Mix (Bio-Rad) on a CFX96 Real-Time PCR System

Notably, only one hGM group (O15) acquired the TMA/TMAO-producing ability from its donor, whereas the other three hGM groups had undetectable plasma d9-TMA and d9-TMAO (Fig

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