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To test whether increased senescence in immune cells from the kidney marrow contributed to the cellular activation in young versus aged killifish, we treated cells from young and aged killifish with a senolytic (fisetin) before immune cell stimulation using LPS

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Production of reactive oxygen species (ROS) was monitored following 5-(and-6)-chloromethyl-2,7-dichlorodihydrofluorescein diacetate acetyl ester and MitoSOX Redderived fluorescence

By incorporating emerging strategies for targeting lymph nodes (LNs) or cellular subsets and subcellular locations, nanoparticle vaccine efficacies can be improved and their immune profiles tailored to address specific diseases