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Beginning with the treatment area being meticulously cleaned and disinfected to ensure a sterile and infection-free environment

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Huh7.5 cells were grown in 3 cm dishes for 24 h, washed with cold serum-free DMEM and maintained for 10 min at 4C before replacing the medium with 1 ml cold serum with or without DMEM and 1 g of LPL, and incubating for a further 20 min at 4C

Overview The structure of bilayers formed by amphipathic lipid molecules has been the subject of intense study since the pioneering work on the nature of the biological membrane by Danielli, Davson, and Robertson in the first half of the 20th century