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Each RNA sample was reverse-transcribed into cDNA with a commercially available cDNA synthesis kit, utilizing Revert Aid H Minus Reverse Transcriptase (Fermentas, Thermo Fisher Scientific Inc., Canada) according to the manufacturers instructions

Zhang YK, Yeager RL, Tanaka Y, Klaassen CD

Genetics, diet, exercise, starting weight, metabolic health, and adherence all influence individual outcomes

Triple therapy has the potential to further decrease COPD exacerbations and hospitalizations versus dual bronchodilator therapy (LABA/LAMA)

Other systemic and metabolic diseases such as type I diabetes can also be influenced by barrier changes [6, 7]