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The Met hydroxy analogs 2-hydroxy-4-(methylthio) butanoic acid (called HMB or HMTBa) and isopropyl ester of 2-hydroxy-4-(methylthio) butanoic acid (HMBi) have been widely used as a precursor of methionine in ruminant nutrition (Noftsger et al., 2003)

Once anesthetized, the right knee joints of the animals were shaved, sterilized, and subjected to an intra-articular injection of collagenase type II