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These include vitamin B 12 deficiency, mutations of the MTHFR gene, and epigenetic modifications that alter expression of other key enzymes involved in folate metabolism

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For four of these metabolites (homotaurine, N -acetyl-phenylalanylleucine/isoleucine, 3-hydroxypropane-1-sulfonic acid, and N 1 -methyl-imidazolelactic acid), the chemical standard did not match to the original tissue peak, but instead matched a peak with a distinct MS/MS spectrum and/or a retention time difference that could not be explained by chromatographic drift (see also Success rate of metabolite discovery)

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Mass range was set to 3501400